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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84516-7-RR (same clone as 84516-7-PBS)

    WB analysis using 84516-7-RR (same clone as 84516-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84516-7-RR (SAE1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84516-7-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 84516-7-RR (same clone as 84516-7-PBS)

    FC experiment of HeLa using 84516-7-RR (same clone as 84516-7-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SAE1 Recombinant antibody (84516-7-RR, Clone:241923C6) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84516-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84516-7-RR

    Affinity and Kinetic Characterization of 84516-7-RR

    Biolayer interferometry (BLl) kinetic assays of 84516-7-RR against Human SAE1 were performed. The affinity constant is 1.98 nM.

SAE1 Recombinant antibody, PBS Only

SAE1 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84516-7-PBS

产品说明书

CloneNo. 241923C6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, FC (Intra), Indirect ELISA

别名

SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84516-7-PBS targets SAE1 in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SAE1 fusion protein Ag0275 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SUMO1 activating enzyme subunit 1
别名 SUMO-activating enzyme subunit 1, SUA1, Sentrin/SUMO activating protein AOS1, HSPC140, AOS1
计算分子量 38 kDa
观测分子量38 kDa
GenBank蛋白编号BC000344
基因名称 SAE1
Gene ID (NCBI) 10055
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UBE0
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 has a calculated molecular mass of 38 kD. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins(PMID:9920803). Western blot analysis of synchronized HeLa cells detectes increased AOS1 expression as cells progressed through S phase, followed by a substantial decrease in G2 phase. Immunofluorescence analysis shows AOS1 and UBA2 distributed throughout nuclei, but they are excluded from nucleoli.(PMID:11481243).