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SAR1A Recombinant antibody, PBS Only

SAR1A Uni-rAbTM Recombinant Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

230471A1

Cat no : 83168-7-PBS

Print datasheet

Synonyms

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

验证数据展示

  • FC experiment of A549 using 83168-7-RR (same clone as 83168-7-PBS)

    FC experiment of A549 using 83168-7-RR (same clone as 83168-7-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

  • FC experiment of A431 using 83168-7-RR (same clone as 83168-7-PBS)

    FC experiment of A431 using 83168-7-RR (same clone as 83168-7-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 83168-7-RR (same clone as 83168-7-PBS)

    IF Staining of HepG2 using 83168-7-RR (same clone as 83168-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (83168-7-RR, Clone: 230471A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 83168-7-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.

产品信息

83168-7-PBS targets SAR1A in IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SAR1 homolog A (S. cerevisiae)
Synonyms SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase
Calculated Molecular Weight 22 kDa
GenBank Accession NumberNM_020150
Gene Symbol SAR1A
Gene ID (NCBI) 56681
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NR31
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).