• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • FC experiment of A549 using 83168-7-RR

    FC experiment of A549 using 83168-7-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of A431 using 83168-7-RR

    FC experiment of A431 using 83168-7-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug SAR1A Recombinant antibody (83168-7-RR, Clone:230471A1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using 83168-7-RR

    IF Staining of HepG2 using 83168-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using SAR1A antibody (83168-7-RR, Clone: 230471A1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

SAR1A Recombinant antibody

SAR1A Recombinant Antibody for IF/ICC, ELISA
Cat No. 83168-7-RR

产品说明书

CloneNo. 230471A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, ELISA

别名

SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inA549 cells, A431 cells
Planning an IHC experiment? We recommend our IHCeasy SAR1A Ready-To-Use IHC Kit. SAR1A primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83168-7-RR targets SAR1A in IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SAR1 homolog A (S. cerevisiae)
别名 SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase
计算分子量 22 kDa
GenBank蛋白编号NM_020150
基因名称 SAR1A
Gene ID (NCBI) 56681
RRIDAB_3670864
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9NR31
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).

实验方案

Product Specific Protocols
IF protocol for SAR1A antibody 83168-7-RRDownload protocol
FC protocol for SAR1A antibody 83168-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols