验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells |
Positive FC (Intra) detected in | A549 cells, A431 cells |
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
83168-7-RR targets SAR1A in IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | SAR1 homolog A (S. cerevisiae) |
Synonyms | SAR1, GTP binding protein SAR1a, EC:3.6.5.2, COPII-associated small GTPase, COPII associated small GTPase |
Calculated Molecular Weight | 22 kDa |
GenBank Accession Number | NM_020150 |
Gene Symbol | SAR1A |
Gene ID (NCBI) | 56681 |
RRID | AB_3670864 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purfication |
UNIPROT ID | Q9NR31 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SAR1A, also named as SAR1, SARA and SARA1, belongs to the small GTPase superfamily and SAR1 family. It is involved in transport from the endoplasmic reticulum to the Golgi apparatus. SAR1A is required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES).
实验方案
Product Specific Protocols | |
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IF protocol for SAR1A antibody 83168-7-RR | Download protocol |
FC protocol for SAR1A antibody 83168-7-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |