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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SCP2 Polyclonal antibody

SCP2 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-23006

Print datasheet

Synonyms

SCP2/SCPx, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16

验证数据展示

  • FC experiment of HepG2 using CL488-23006

    FC experiment of HepG2 using CL488-23006

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Scp2 Polyclonal Antibody (CL488-23006)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 23006-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-23006 targets SCP2 in FC (Intra) applications and shows reactivity with human samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human
Immunogen SCP2 fusion protein Ag19215 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sterol carrier protein 2
Synonyms SCP2/SCPx, NSL-TP, Non-specific lipid-transfer protein, EC:2.3.1.176, EC:2.3.1.16
Calculated Molecular Weight 547 aa, 59 kDa
Observed Molecular Weight 13-15 kDa
GenBank Accession NumberBC005911
Gene Symbol SCP2
Gene ID (NCBI) 6342
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22307
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SCP2 (Sterol carrier protein-2), also called nonspecific lipid-transfer protein, is thought to play a major role in intracellular lipid transport and metabolism. Mutations of SCP2 have been associated with diseases involving abnormalities in lipid trafficking, such as Zellweger syndrome. The alternative splicing generates 58 kDa SCPx and 13-15 kDa SCP2 proteins, both of which contain a C-terninal SCP2 domain. In addition to the SCP2 domain, SCPx also contains an amino-terminal thiolase domain. The proteolytic cleavage of SCPx occurred when it was imported into peroxisomes, giving rise to a 44-46 kDa thiolase and a 13-15 kDa SCP2. 23006-1-AP antibody recognizes the 13-15 kDa SCP2 protein.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SCP2 antibody CL488-23006Download protocol
Standard Protocols
Click here to view our Standard Protocols