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验证数据展示

  • FC experiment of U266 using 98051-1-PBS

    FC experiment of U266 using 98051-1-PBS

    1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only (98051-1-PBS, Clone: 240422C4) (red) or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

  • FC experiment of U266 using 98051-1-RR (same clone as 98051-1-PBS)

    FC experiment of U266 using 98051-1-RR (same clone as 98051-1-PBS)

    1x10^6 U266 cells (left) or Jurkat cells (right) were surface stained with 0.25 ug Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody (98051-1-RR, Clone: 240422C4) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (red), or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L) (blue). Cells were not fixed. This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    IHC staining of human tonsillitis using 98051-1-RR (same clone as 98051-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98051-1-RR (CD138/Syndecan-1 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 98051-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CD138/Syndecan-1 antibody included
  • Affinity and Kinetic Characterization of 98051-1-RR

    Affinity and Kinetic Characterization of 98051-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98051-1-RR against Human CD138/Syndecan-1 were performed. The affinity constant is 20.8 nM.

Anti-Human CD138/Syndecan-1 Rabbit Recombinant Antibody, PBS Only

CD138/Syndecan-1 Uni-rAb® Recombinant Antibody for IHC, FC
Cat No. 98051-1-PBS

产品说明书

CloneNo. 240422C4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, FC

别名

CD138, 240422C4, syndecan, SYND1, SDC1

缓冲液配方:  PBS Only
PBS, Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive FC detected inU266 cells
Planning an IHC experiment? We recommend our IHCeasy CD138/Syndecan-1 Ready-To-Use IHC Kit. CD138/Syndecan-1 primary antibody included.

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in 100 μl suspension
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98051-1-PBS targets CD138/Syndecan-1 in IHC, FC applications and shows reactivity with human samples.

经测试应用 IHC, FC Application Description
经测试反应性 human
免疫原

Recombinant protein

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 syndecan 1
别名 CD138, 240422C4, syndecan, SYND1, SDC1
计算分子量 32.5 kDa
GenBank蛋白编号BC008765
基因名称 CD138
Gene ID (NCBI) 6382
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDP18827
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.

实验方案

Product Specific Protocols
FC protocol for CD138/Syndecan-1 antibody 98051-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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