验证数据展示
经过测试的应用
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | U266 cells |
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
98051-1-RR targets CD138/Syndecan-1 in IHC, FC applications and shows reactivity with human samples.
| 经测试应用 | IHC, FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Recombinant protein 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | syndecan 1 |
| 别名 | CD138, 240422C4, syndecan, SYND1, SDC1 |
| 计算分子量 | 32.5 kDa |
| GenBank蛋白编号 | BC008765 |
| 基因名称 | CD138 |
| Gene ID (NCBI) | 6382 |
| RRID | AB_3672198 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P18827 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Stable for one year after shipment. |
背景介绍
CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD138/Syndecan-1 antibody 98051-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |