验证数据展示
经过测试的应用
| Positive WB detected in | L02 cells, HuH-7 cells, HepG2 cells, A549 cells, NCI-H1299 cells, Jurkat cells |
| Positive IHC detected in | human liver cancer tissue, human colon cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67088-1-Ig targets SDC2 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IHC |
| 经测试反应性 | Human |
| 文献引用反应性 | human, pig |
| 免疫原 | SDC2 fusion protein Ag5739 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syndecan 2 |
| 别名 | Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 19 kDa, 22 and 48 kDa |
| GenBank蛋白编号 | BC049836 |
| 基因名称 | SDC2 |
| Gene ID (NCBI) | 6383 |
| RRID | AB_2882395 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P34741 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SDC2 antibody 67088-1-Ig | Download protocol |
| IHC protocol for SDC2 antibody 67088-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Oncol (Dordr) Circular RNA circNFATC3 acts as a miR-9-5p sponge to promote cervical cancer development by upregulating SDC2. | ||
J Cell Mol Med miR-218 affects the ECM composition and cell biomechanical properties of glioblastoma cells. | ||
Cancer Cell Int Identification of an immune-related signature indicating the dedifferentiation of thyroid cells. | ||
Cancer Cell Int GNG5 is a novel oncogene associated with cell migration, proliferation, and poor prognosis in glioma. | ||
Heart Vessels Downregulation of lncRNA SNHG16 inhibits vascular smooth muscle cell proliferation and migration in cerebral atherosclerosis by targeting the miR-30c-5p/SDC2 axis. |
