验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HuH-7 cells, L02 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67088 targets SDC2 in WB applications and shows reactivity with Human samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | Human |
| 免疫原 | SDC2 fusion protein Ag5739 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | syndecan 2 |
| 别名 | Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2 |
| 计算分子量 | 22 kDa |
| 观测分子量 | 22 kDa |
| GenBank蛋白编号 | BC049836 |
| 基因名称 | SDC2 |
| Gene ID (NCBI) | 6383 |
| RRID | AB_3084307 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P34741 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 SDC2 antibody CL488-67088 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |