CoraLite® Plus 488-conjugated SDC2 Monoclonal antibody
SDC2 Monoclonal Antibody for WB
Host / Isotype
Mouse / IgG2a
Reactivity
Human
Applications
WB
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
3A7B2
验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HuH-7 cells, L02 cells |
For other applications, we recommend the unconjugated version of this antibody, 67088-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67088 targets SDC2 in WB applications and shows reactivity with Human samples.
| Tested Applications | WB Application Description |
| Tested Reactivity | Human |
| Immunogen | SDC2 fusion protein Ag5739 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | syndecan 2 |
| Synonyms | Fibroglycan, HSPG, HSPG1, SDC2, SYND2, syndecan 2 |
| Calculated Molecular Weight | 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC049836 |
| Gene Symbol | SDC2 |
| Gene ID (NCBI) | 6383 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P34741 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDC2, containing cell surface heparan sulphate proteoglycan, functions as an integral membrane protein and participates in cell proliferation, cell migration, cell adhesion and signal transduction. It has been reported that the expression level of SDC2 will be up-regulated under hypoxia condition which can induce increased release of cytokines and chemokines. Besides, methylated SDC2 can be used as a potential biomarker for early diagnosis of colon cancer. 67088-1-Ig antibody detects the 19 and 22 kDa SDC2 isoforms as well as the 48 kDa dimeric protein in SDS-PAGE.(PMID: 18803305, 23297089, 30022330, 29945573)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 488 SDC2 antibody CL488-67088 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |