验证数据展示
经过测试的应用
| Positive IF/ICC detected in | MDCK cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-13471 targets SDCCAG8 in IF/ICC applications and shows reactivity with human, mouse, rat, zebrafish, Canine samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human, mouse, rat, zebrafish, Canine |
| 免疫原 | SDCCAG8 fusion protein Ag4264 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | serologically defined colon cancer antigen 8 |
| 别名 | Antigen NY CO 8, BBS16, CCCAP, hCCCAP, HSPC085, NY CO 8, SDCCAG8 |
| 计算分子量 | 713 aa, 83 kDa |
| GenBank蛋白编号 | BC032454 |
| 基因名称 | SDCCAG8 |
| Gene ID (NCBI) | 10806 |
| RRID | AB_2919072 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q86SQ7 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDCCAG8, also named as CCCAP and NY-CO-8, plays a role in the establishment of cell polarity and epithelial lumen formation. It may play a role in ciliogenesis. Loss of SDCCAG8 function as a cause of a retinal-renal ciliopathy and validates exome capture analysis for broadly heterogeneous single-gene disorders. SDCCAG8 is localized at both centrioles and interacts directly with OFD1 (oral-facial-digital syndrome 1), which is associated with NPHP-RC. SDCCAG8 has 4 isoforms with MW 83,78,73 and 41 kDa (refer to UniProt). Catalog#13471-1-AP can recognize all the four isoforms.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SDCCAG8 antibody CL488-13471 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |