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验证数据展示

  • IF Staining of HepG2 using CL488-66588

    IF Staining of HepG2 using CL488-66588

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488-conjugated SDHA antibody (CL488-66588, Clone: 5E10G12 ) at dilution of 1:100. Red: CL594-Phalloidin stains F-Actin; Blue: DAPI.

  • IF Staining of HepG2 using CL488-66588

    IF Staining of HepG2 using CL488-66588

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66588 (SDHA antibody) at dilution of 1:100.

  • FC experiment of HeLa using CL488-66588

    FC experiment of HeLa using CL488-66588

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SDHA (CL488-66588, Clone:5E10G12) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SDHA Monoclonal antibody

SDHA Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66588

产品说明书

CloneNo. 5E10G12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SDHA Ready-To-Use IHC Kit. SDHA primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66588-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66588 targets SDHA in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SDHA fusion protein Ag7023 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 succinate dehydrogenase complex, subunit A, flavoprotein (Fp)
别名 PPGL5, PGL5, MC2DN1, FP, EC:1.3.5.1
计算分子量 73 kDa
观测分子量 62-73 kDa
GenBank蛋白编号BC001380
基因名称 SDHA
Gene ID (NCBI) 6389
RRIDAB_2883359
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31040
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SDHA antibody CL488-66588Download protocol
FC protocol for CL Plus 488 SDHA antibody CL488-66588Download protocol
Standard Protocols
Click here to view our Standard Protocols