验证数据展示
经过测试的应用
Positive IF/ICC detected in | HepG2 cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL594-66588 targets SDHA in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | IF/ICC Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | SDHA fusion protein Ag7023 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | succinate dehydrogenase complex, subunit A, flavoprotein (Fp) |
别名 | FP, SDH2, SDHA, SDHF |
计算分子量 | 73 kDa |
观测分子量 | 62-73 kDa |
GenBank蛋白编号 | BC001380 |
基因名称 | SDHA |
Gene ID (NCBI) | 6389 |
RRID | AB_2883606 |
偶联类型 | CoraLite®594 Fluorescent Dye |
最大激发/发射波长 | 588 nm / 604 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P31040 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDHA, also named as SDH2, SDHF and Fp, belongs to the FAD-dependent oxidoreductase 2 family and FRD/SDH subfamily. SDHA is the flavoprotein subunit of succinate dehydrogenase (SDH) that is involved in complex II of the mitochondrial electron transport chain and is responsible for transferring electrons from succinate to ubiquinone (coenzyme Q). SDHA has 3 isoforms with the molecular mass of 73, 67 and 57 kDa, and the N-terminal (~5 kDa) can be removed in mature form. It can be detected a band of 68 kDa in cutaneous melanoma(PMID:21698147). SDHA may act as a tumor suppressor.
实验方案
Product Specific Protocols | |
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IF protocol for CL594 SDHA antibody CL594-66588 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |