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SDHB Monoclonal antibody

SDHB Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67600-1-Ig

产品说明书

CloneNo. 1A2A5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig and More (1)

应用

WB, IHC, IF-P, ELISA

SDH, PGL4, PGL 4, Iron-sulfur subunit of complex II, EC:1.3.5.1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inU2OS cells, HeLa cells, HepG2 cells, HSC-T6 cells, pig brain tissue, HEK-293 cells, K-562 cells, NIH/3T3 cells, Neuro-2a cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse skeletal muscle tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman liver cancer tissue
Planning an IHC experiment? We recommend our IHCeasy SDHB Ready-To-Use IHC Kit. SDHB primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 5 publications below

产品信息

67600-1-Ig targets SDHB in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse, rat, pig
文献引用反应性human, mouse, rabbit
免疫原 SDHB fusion protein Ag29868 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
别名 SDH, PGL4, PGL 4, Iron-sulfur subunit of complex II, EC:1.3.5.1
计算分子量 32 kDa
观测分子量 28-32 kDa
GenBank蛋白编号BC007840
基因名称 SDHB
Gene ID (NCBI) 6390
RRIDAB_2882806
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP21912
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).

实验方案

Product Specific Protocols
WB protocol for SDHB antibody 67600-1-IgDownload protocol
IHC protocol for SDHB antibody 67600-1-IgDownload protocol
IF protocol for SDHB antibody 67600-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Phytomedicine

Zhen Wu decoction represses renal fibrosis by invigorating tubular NRF2 and TFAM to fuel mitochondrial bioenergetics

Authors - Min Zheng
mouseWB

Phytother Res

Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels.

Authors - Long Cheng
mouseWB

Chin J Nat Med

Mulberry leaf flavonoids activate BAT and induce browning of WAT to improve type 2 diabetes via regulating the AMPK/SIRT1/PGC-1α signaling pathway

Authors - Long Cheng
rabbitWB

Int J Biol Macromol

Plasma-derived exosome miR-10a-5p promotes premature ovarian failure by target BDNF via the TrkB/Akt/mTOR signaling pathway

Authors - Zhiyuan Bao
humanWB

Biochim Biophys Acta Mol Basis Dis

LARP1, an RNA-binding protein, participates in ovarian cancer cell survival by regulating mitochondrial oxidative phosphorylation in response to the influence of the PI3K/mTOR pathway

Authors - Jiaoyan Ma
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