验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67600 targets SDHB in IF-P applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | IF-P Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | SDHB fusion protein Ag29868 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | succinate dehydrogenase complex, subunit B, iron sulfur (Ip) |
| 别名 | FLJ92337, IP, PGL4, SDH, SDH1, SDHB, SDHIP |
| 计算分子量 | 32 kDa |
| 观测分子量 | 28-32 kDa |
| GenBank蛋白编号 | BC007840 |
| 基因名称 | SDHB |
| Gene ID (NCBI) | 6390 |
| RRID | AB_2920162 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P21912 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 SDHB antibody CL594-67600 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |