- Featured Product
- KD/KO Validated
CoraLite® Plus 488-conjugated SDHB Monoclonal antibody
SDHB Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
1A2A5
验证数据展示
经过测试的应用
| Positive IF-P detected in | human liver cancer tissue |
For other applications, we recommend the unconjugated version of this antibody, 67600-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67600 targets SDHB in IF-P applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human, Mouse, Rat |
| Immunogen | SDHB fusion protein Ag29868 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | succinate dehydrogenase complex, subunit B, iron sulfur (Ip) |
| Synonyms | FLJ92337, IP, PGL4, SDH, SDH1, SDHB, SDHIP |
| Calculated Molecular Weight | 32 kDa |
| Observed Molecular Weight | 28-32 kDa |
| GenBank Accession Number | BC007840 |
| Gene Symbol | SDHB |
| Gene ID (NCBI) | 6390 |
| RRID | AB_2919505 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P21912 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SDHB(Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial) is also named as SDH, SDH1 and belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. The SDHD, SDHB, and SDHC genes encode subunits of mitochondrial complex II (succinate dehydrogenase). Mitochondrial complex II is a heterotetrameric complex involved in the aerobic electron transport chain and catalyses the oxidation of succinate to fumarate (Krebs cycle) with transport of electrons to the ubiquinone pool. SDHA and SDHB are the hydrophilic catalytic part of the complex and are highly conserved(J Med Genet 2004;41:e99). Defects in SDHB are a cause of susceptibility to pheochromocytoma (PCC), paragangliomas type 4 (PGL4), paraganglioma and gastric stromal sarcoma (PGGSS) and Cowden-like syndrome (CWDLS).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 SDHB antibody CL488-67600 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |