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SERPINE2 Recombinant antibody, PBS Only

SERPINE2 Uni-rAbTM Recombinant Antibody for Indirect ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

5O16

Cat no : 82732-5-PBS

Print datasheet

Synonyms

PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7

验证数据展示

  • WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    WB analysis using 82732-5-RR (same clone as 82732-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 82732-5-RR (same clone as 82732-5-PBS)

    FC experiment of A549 using 82732-5-RR (same clone as 82732-5-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82732-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82732-5-RR

    Affinity and Kinetic Characterization of 82732-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

产品信息

82732-5-PBS targets SERPINE2 in Indirect ELISA, WB applications and shows reactivity with rat, mouse, human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity rat, mouse, human
Immunogen SERPINE2 fusion protein Ag1830 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
Synonyms PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7
Calculated Molecular Weight 44 kDa
Observed Molecular Weight 44-50 kDa
GenBank Accession NumberBC015663
Gene Symbol SERPINE2
Gene ID (NCBI) 5270
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07093
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.