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验证数据展示

  • WB analysis using 82732-5-RR

    WB analysis using 82732-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82732-5-RR

    WB analysis using 82732-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82732-5-RR (SERPINE2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • FC experiment of A549 using 82732-5-RR

    FC experiment of A549 using 82732-5-RR

    1x10^6 A549 cells were intracellularly stained with 0.25 ug SERPINE2 Recombinant antibody (82732-5-RR, Clone:5O16) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82732-5-RR

    Affinity and Kinetic Characterization of 82732-5-RR

    Biolayer interferometry (BLl) kinetic assays of 82732-5-RR against Human SERPINE2 were performed. The affinity constant is below 1 pM.

SERPINE2 Recombinant antibody

SERPINE2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 82732-5-RR

产品说明书

CloneNo. 5O16

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inU-87 MG cells, C6 cells, U-251 cells, mouse brain tissue, rat brain tissue
Positive FC (Intra) detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82732-5-RR targets SERPINE2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SERPINE2 fusion protein Ag1830 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2
别名 PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7
计算分子量 44 kDa
观测分子量 44-50 kDa
GenBank蛋白编号BC015663
基因名称 SERPINE2
Gene ID (NCBI) 5270
RRIDAB_3670540
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.

实验方案

Product Specific Protocols
WB protocol for SERPINE2 antibody 82732-5-RRDownload protocol
FC protocol for SERPINE2 antibody 82732-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols