验证数据展示
经过测试的应用
Positive WB detected in | U-87 MG cells, C6 cells, U-251 cells, mouse brain tissue, rat brain tissue |
Positive FC (Intra) detected in | A549 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
82732-5-RR targets SERPINE2 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, FC (Intra), ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | SERPINE2 fusion protein Ag1830 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 2 |
Synonyms | PI7, PN1, Serpin E2, PI 7, Peptidase inhibitor 7 |
Calculated Molecular Weight | 44 kDa |
Observed Molecular Weight | 44-50 kDa |
GenBank Accession Number | BC015663 |
Gene Symbol | SERPINE2 |
Gene ID (NCBI) | 5270 |
RRID | AB_3670540 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P07093 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPINE2 also called protease nexin-1 or glial-derived neurite promoting factor, is a member of the serine protease inhibitor (SERPIN) superfamily. It would be a potent inhibitor of the urokinase-plasminogen activator (uPA), tissue-type PA (tPA), thrombin, trypsin, factor Xia, and prostasin. SERPINE2 is widely expressed in various tissues, including endothelial cells, fibroblasts, smooth muscle cells, tumor cells, glial cells, neurons, and placental cells.
实验方案
Product Specific Protocols | |
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WB protocol for SERPINE2 antibody 82732-5-RR | Download protocol |
FC protocol for SERPINE2 antibody 82732-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |