验证数据展示
经过测试的应用
| Positive WB detected in | human plasma tissue, human plasma, pig plasma, rat serum |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy SERPING1 Ready-To-Use IHC Kit. SERPING1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
66882-1-Ig targets SERPING1/C1 Inactivator in WB, IF/ICC, ELISA applications and shows reactivity with Human, pig, rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, pig, rat |
| 文献引用反应性 | human |
| 免疫原 | SERPING1/C1 Inactivator fusion protein Ag16782 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2a |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade G (C1 inhibitor), member 1 |
| 别名 | C1 esterase inhibitor, C1 Inh, C1 inhibiting factor, C1IN, C1INH, C1NH, HAE1, HAE2, Plasma protease C1 inhibitor, Serpin G1, SERPING1, SERPING1/C1 Inactivator |
| 计算分子量 | 500 aa, 55 kDa |
| 观测分子量 | 100 kDa |
| GenBank蛋白编号 | BC011171 |
| 基因名称 | SERPING1 |
| Gene ID (NCBI) | 710 |
| RRID | AB_2882214 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05155 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SERPING1, also known as C1 Inhibitor (C1INH), is a member of the Serine proteinase inhibitor family. Its main function is the inhibition of the complement system to prevent spontaneous activation. It inhibits activated C1r and C1s of the first complement component and thus regulates complement activation. Deficiency of this protein is associated with hereditary angioneurotic oedema (HANE). C1INH is the most heavily glycosylated plasma protein.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SERPING1/C1 Inactivator antibody 66882-1-Ig | Download protocol |
| IF protocol for SERPING1/C1 Inactivator antibody 66882-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
