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SHBG Recombinant antibody

SHBG Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230356F10

Cat No : 83096-1-RR

Print datasheet

Synonyms

Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP

验证数据展示

  • WB analysis of human placenta using 83096-1-RR

    WB analysis of human placenta using 83096-1-RR

    human placenta tissue were subjected to SDS PAGE followed by western blot with 83096-1-RR (SHBG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83096-1-RR

    FC experiment of HeLa using 83096-1-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SHBG Recombinant antibody (83096-1-RR, Clone:230356F10) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83096-1-RR

    Affinity and Kinetic Characterization of 83096-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83096-1-RR against Human SHBG were performed. The affinity constant is 0.38 nM.

经过测试的应用

Positive WB detected inhuman placenta tissue
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83096-1-RR targets SHBG in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name sex hormone-binding globulin
Synonyms Testis-specific androgen-binding protein, Sex steroid-binding protein, Sex steroid binding protein, Sex hormone-binding globulin, SBP
Calculated Molecular Weight 402 aa, 44 kDa
Observed Molecular Weight40-50 kDa
GenBank Accession NumberBC101785
Gene Symbol SHBG
Gene ID (NCBI) 6462
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04278
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SHBG (sex hormone-binding globulin), also known as androgen-binding protein (ABP), is the specific transport protein for sex steroid hormones in humans. SHBG is synthesized mainly by the liver and circulates in the blood, in form of homodimer with a single steroid-binding site per dimer. In addition to the plasma SHBG, a testis isoform expressed by Sortoli cells also exists due to the gene alternative splicing. Various bands (44-56 kDa) recognized by this antibody may represent variably glycosylated or different isoforms of SHBG.

实验方案

Product Specific Protocols
WB protocol for SHBG antibody 83096-1-RRDownload protocol
FC protocol for SHBG antibody 83096-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols