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SIAE Monoclonal antibody

SIAE Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1G5F7

Cat no : 66522-1-Ig

Print datasheet

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

验证数据展示

  • WB analysis using 66522-1-Ig

    WB analysis using 66522-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig colon using 66522-1-Ig

    WB analysis of pig colon using 66522-1-Ig

    pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-3 using 66522-1-Ig

    IF Staining of PC-3 using 66522-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inpig liver tissue, pig colon tissue, rat colon tissue, mouse liver tissue
Positive IF/ICC detected inPC-3 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66522-1-Ig targets SIAE in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SIAE fusion protein Ag14624 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sialic acid acetylesterase
Synonyms Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC068450
Gene Symbol SIAE
Gene ID (NCBI) 54414
RRIDAB_2881885
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HAT2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SIAE antibody 66522-1-IgDownload protocol
IF protocol for SIAE antibody 66522-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols