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CoraLite® Plus 488-conjugated SIAE Monoclonal antibody

SIAE Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G5F7

Cat no : CL488-66522

Print datasheet

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

验证数据展示

  • IF Staining of PC-3 using CL488-66522

    IF Staining of PC-3 using CL488-66522

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using CoraLite® Plus 488 SIAE antibody (CL488-66522, Clone: 1G5F7 ) at dilution of 1:600, CL594-Phalloidin (red).

  • FC experiment of PC-3 using CL488-66522

    FC experiment of PC-3 using CL488-66522

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SIAE (CL488-66522, Clone:1G5F7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inPC-3 cells
Positive FC (Intra) detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 66522-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:300-1:1200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66522 targets SIAE in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SIAE fusion protein Ag14624 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name sialic acid acetylesterase
Synonyms Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53
Calculated Molecular Weight 58 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC068450
Gene Symbol SIAE
Gene ID (NCBI) 54414
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9HAT2
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SIAE antibody CL488-66522Download protocol
FC protocol for CL Plus 488 SIAE antibody CL488-66522Download protocol
Standard Protocols
Click here to view our Standard Protocols