SIAE Monoclonal antibody, PBS Only

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1G5F7

Cat no : 66522-1-PBS

Synonyms

Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53

WB analysis using 66522-1-Ig (same clone as 66522-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.
WB analysis of pig colon using 66522-1-Ig (same clone as 66522-1-PBS)
pig colon tissue were subjected to SDS PAGE followed by western blot with 66522-1-Ig (SIAE antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 66522-1-Ig (same clone as 66522-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using SIAE antibody (66522-1-Ig, Clone: 1G5F7 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66522-1-PBS in a different storage buffer formulation.

Size

66522-1-PBS targets SIAE in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications	WB, IF/ICC, Indirect ELISA Application Description
Tested Reactivity	human, mouse, rat, pig
Immunogen	SIAE fusion protein Ag14624 种属同源性预测
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Full Name	sialic acid acetylesterase
Synonyms	Sialate O-acetylesterase, LSE, H-Lse, H Lse, EC:3.1.1.53
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC068450
Gene Symbol	SIAE
Gene ID (NCBI)	54414
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	Q9HAT2
Storage Buffer	PBS Only
Storage Conditions	Store at -80°C. The product is shipped with ice packs. Upon receipt, store it immediately at -80°C