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CoraLite®532-conjugated SIRP Alpha/CD172a Recombinant antibody

SIRP Alpha/CD172a Uni-rAbTM Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®532 Fluorescent Dye

CloneNo.

240789C6

Cat no : CL532-83729-5

Print datasheet

Synonyms

SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A

验证数据展示

  • IF Staining of THP-1 using CL532-83729-5

    IF Staining of THP-1 using CL532-83729-5

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using CoraLite®532 SIRP Alpha/CD172a antibody (CL532-83729-5, Clone: 240789C6 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inTHP-1 cells
For other applications, we recommend the unconjugated version of this antibody, 83729-5-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of CL532-83729-5 is SIRP Alpha/CD172a Fusion Protein expressed in E. coli.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen Fusion Protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal-regulatory protein alpha
Synonyms SIRP Alpha, SIRPA, Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A
Calculated Molecular Weight55 kDa
Observed Molecular Weight 75-85 kDa
GenBank Accession NumberNM_080792
Gene Symbol SIRP Alpha
Gene ID (NCBI) 140885
Conjugate CoraLite®532 Fluorescent Dye
Excitation/Emission Maxima Wavelengths537 nm / 560 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78324
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).

实验方案

Product Specific Protocols
IF protocol for CL532 SIRP Alpha/CD172a antibody CL532-83729-5Download protocol
Standard Protocols
Click here to view our Standard Protocols