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验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98267-1-RR

    FC experiment of human peripheral blood leukocytes using 98267-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody (98267-1-RR, Clone: 242115C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98267-1-RR

    FC experiment of human peripheral blood leukocytes using 98267-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody (98267-1-RR, Clone: 242115C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98267-1-RR

    Affinity and Kinetic Characterization of 98267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98267-1-RR against Human SIRP beta 1/CD172b were performed. The affinity constant is 22.8 pM.

Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody

SIRP beta 1/CD172b Uni-rAbTM Recombinant Antibody for FC
Cat No. 98267-1-RR

产品说明书

CloneNo. 242115C10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC

别名

SIRP BETA 1, SIRPB1, Signal-regulatory protein beta-1 isoform 3, Signal-regulatory protein beta-1, CD172b

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

应用推荐稀释比
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98267-1-RR targets SIRP beta 1/CD172b in FC applications and shows reactivity with human samples.

经测试应用 FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 signal-regulatory protein beta 1
别名 SIRP BETA 1, SIRPB1, Signal-regulatory protein beta-1 isoform 3, Signal-regulatory protein beta-1, CD172b
计算分子量43kDa
GenBank蛋白编号NM_006065.5
基因名称 SIRPB1
Gene ID (NCBI) 10326
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDO00241-1
储存缓冲液 PBS with 0.09% sodium azide , pH 7.3
储存条件Store at 2 - 8°C. Stable for one year after shipment.

背景介绍

SIRP beta 1, also known as CD172b, is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP beta 1 also participates in the recruitment of tyrosine kinase SYK.

实验方案

Product Specific Protocols
FC protocol for SIRP beta 1/CD172b antibody 98267-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols