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Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody

SIRP beta 1/CD172b Uni-rAbTM Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

242115C10

Cat no : 98267-1-RR

Print datasheet

Synonyms

SIRP BETA 1, SIRPB1, Signal-regulatory protein beta-1 isoform 3, Signal-regulatory protein beta-1, CD172b

验证数据展示

  • FC experiment of human peripheral blood leukocytes using 98267-1-RR

    FC experiment of human peripheral blood leukocytes using 98267-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody (98267-1-RR, Clone: 242115C10) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD14. Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • FC experiment of human peripheral blood leukocytes using 98267-1-RR

    FC experiment of human peripheral blood leukocytes using 98267-1-RR

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human SIRP beta 1/CD172b Rabbit Recombinant Antibody (98267-1-RR, Clone: 242115C10) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

  • Affinity and Kinetic Characterization of 98267-1-RR

    Affinity and Kinetic Characterization of 98267-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98267-1-RR against Human SIRP beta 1/CD172b were performed. The affinity constant is 22.8 pM.

经过测试的应用

Positive FC detected inhuman peripheral blood leukocytes

推荐稀释比

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

98267-1-RR targets SIRP beta 1/CD172b in FC applications and shows reactivity with human samples.

Tested Applications FC Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal-regulatory protein beta 1
Synonyms SIRP BETA 1, SIRPB1, Signal-regulatory protein beta-1 isoform 3, Signal-regulatory protein beta-1, CD172b
Calculated Molecular Weight43kDa
GenBank Accession NumberNM_006065.5
Gene Symbol SIRPB1
Gene ID (NCBI) 10326
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO00241-1
Storage Buffer PBS with 0.09% sodium azide, pH 7.3.
Storage ConditionsStore at 2 - 8°C. Stable for one year after shipment.

背景介绍

SIRP beta 1, also known as CD172b, is a member of the signal-regulatory-protein (SIRP) family and also belongs to the immunoglobulin superfamily. SIRP family members are cell surface signaling receptors differentially expressed in leukocytes and the central nervous system. SIRP beta 1 interacts with TYROBP/DAP12, a protein-bearing immunoreceptor tyrosine-based activation motif. SIRP beta 1 also participates in the recruitment of tyrosine kinase SYK.

实验方案

Product Specific Protocols
FC protocol for SIRP beta 1/CD172b antibody 98267-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols