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验证数据展示

  • WB analysis using 66771-1-Ig

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 66771-1-Ig

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB, ELISA
Cat No. 66771-1-Ig

产品说明书

CloneNo. 1F11A3
引用文献(1)

宿主/亚型

Mouse / IgG3

种属反应性

Human, Mouse, Rat and More (1)

应用

WB, ELISA and More (1)

别名

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, NK-92 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66771-1-Ig targets SLC25A1 in WB, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IP
经测试反应性 Human, Mouse, Rat
文献引用反应性mouse
免疫原 SLC25A1 fusion protein Ag7520 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
别名 Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC004980
基因名称 SLC25A1
Gene ID (NCBI) 6576
RRIDAB_2882117
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53007
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

实验方案

Product Specific Protocols
WB protocol for SLC25A1 antibody 66771-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Nat Commun

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Authors - Weiwei Liu
View Article