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SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG3

Reactivity

Human, Mouse, Rat and More (1)

Applications

WB, ELISA and More (1)

Conjugate

Unconjugated

CloneNo.

1F11A3

Cat no : 66771-1-Ig

Print datasheet

Synonyms

Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein

验证数据展示

  • WB analysis using 66771-1-Ig

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis using 66771-1-Ig

    WB analysis using 66771-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66771-1-Ig (SLC25A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, NK-92 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66771-1-Ig targets SLC25A1 in WB, IP, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, ELISA Application Description
Cited ApplicationsWB, IP
Tested Reactivity Human, Mouse, Rat
Cited Reactivitymouse
Immunogen SLC25A1 fusion protein Ag7520 种属同源性预测
Host / Isotype Mouse / IgG3
Class Monoclonal
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
Synonyms Citrate transport protein, CTP, SLC20A3, SLC25A1, Tricarboxylate carrier protein
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC004980
Gene Symbol SLC25A1
Gene ID (NCBI) 6576
RRIDAB_2882117
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP53007
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

实验方案

Product Specific Protocols
WB protocol for SLC25A1 antibody 66771-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IP

Nat Commun

IL-1R-IRAKM-Slc25a1 signaling axis reprograms lipogenesis in adipocytes to promote diet-induced obesity in mice.

Authors - Weiwei Liu
View Article