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验证数据展示

  • WB analysis using CL488-66771

    WB analysis using CL488-66771

    Various lysates were subjected to SDS PAGE followed by western blot with CL488-66771 (SLC25A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

CoraLite® Plus 488-conjugated SLC25A1 Monoclonal antibody

SLC25A1 Monoclonal Antibody for WB
Cat No. CL488-66771

产品说明书

CloneNo. 1F11A3

宿主/亚型

Mouse / IgG3

种属反应性

human, mouse, rat

应用

WB

别名

Tricarboxylate carrier protein, SLC20A3, CTP, Citrate transport protein, CIC

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
For other applications, we recommend the unconjugated version of this antibody, 66771-1-Ig

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66771 targets SLC25A1 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 SLC25A1 fusion protein Ag7520 种属同源性预测
宿主/亚型 Mouse / IgG3
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 25 (mitochondrial carrier; citrate transporter), member 1
别名 Tricarboxylate carrier protein, SLC20A3, CTP, Citrate transport protein, CIC
计算分子量 34 kDa
观测分子量 34 kDa
GenBank蛋白编号BC004980
基因名称 SLC25A1
Gene ID (NCBI) 6576
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP53007
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SLC25A1, also known as CIC or CTP, is a mitochondrial citrate transporter that exports citrate from the mitochondria to the cytosol. SLC25A1 is highly expressed in several tumor types. Recently it has been identified as a novel transcriptional target of mutant p53 and a negative tumor prognostic marker. In addition, defects in SLC25A1 has been found as a cause of combined D-2- and L-2-hydroxyglutaric aciduria. This antibody specifically recognized the endogenous SLC25A1; no protein was detected in cells from subjects containing two alleles with truncating mutations with this antibody. (24681808, 23561848)

实验方案

Product Specific Protocols
WB protocol for CL Plus 488 SLC25A1 antibody CL488-66771Download protocol
Standard Protocols
Click here to view our Standard Protocols