CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody

SLC25A5 Uni-rAbTM Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230247B1

Cat no : CL750-83104

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Synonyms

ANT1/2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2



经过测试的应用

Positive WB detected inC6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells
Positive FC (Intra) detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy SLC25A5 Ready-To-Use IHC Kit. SLC25A5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83104-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-83104 targets SLC25A5 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC25A5 fusion protein Ag11956 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5
Synonyms ANT1/2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2
Calculated Molecular Weight 298 aa, 33 kDa
Observed Molecular Weight32 kDa
GenBank Accession NumberBC056160
Gene Symbol SLC25A5
Gene ID (NCBI) 292
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05141
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 SLC25A5 antibody CL750-83104Download protocol
Standard Protocols
Click here to view our Standard Protocols