验证数据展示
经过测试的应用
| Positive WB detected in | C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells |
| Positive FC (Intra) detected in | PC-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL750-83104 targets ANT1/2 in WB applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | ANT1/2 fusion protein Ag11956 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 |
| 别名 | SLC25A5, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2 |
| 计算分子量 | 298 aa, 33 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC056160 |
| 基因名称 | SLC25A5 |
| Gene ID (NCBI) | 292 |
| RRID | AB_3673767 |
| 偶联类型 | CoraLite® Plus 750 Fluorescent Dye |
| 最大激发/发射波长 | 755 nm / 780 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P05141 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CL Plus 750 ANT1/2 antibody CL750-83104 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |