CoraLite® Plus 750-conjugated SLC25A5 Recombinant antibody
SLC25A5 Uni-rAbTM Recombinant Antibody for WB
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB
Conjugate
CoraLite® Plus 750 Fluorescent Dye
CloneNo.
230247B1
验证数据展示
经过测试的应用
Positive WB detected in | C6 cells, rat liver tissue, mouse liver tissue, Neuro-2a cells |
Planning an IHC experiment? We recommend our IHCeasy SLC25A5 Ready-To-Use IHC Kit. SLC25A5 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83104-1-PBS
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL750-83104 targets SLC25A5 in WB applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | SLC25A5 fusion protein Ag11956 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | solute carrier family 25 (mitochondrial carrier; adenine nucleotide translocator), member 5 |
Synonyms | ANT1/2, ADP/ATP translocase 2, N-terminally processed, ADP/ATP translocase 2, ADP,ATP carrier protein 2, AAC2 |
Calculated Molecular Weight | 298 aa, 33 kDa |
Observed Molecular Weight | 32 kDa |
GenBank Accession Number | BC056160 |
Gene Symbol | SLC25A5 |
Gene ID (NCBI) | 292 |
Conjugate | CoraLite® Plus 750 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 755 nm / 780 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P05141 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Adenine nucleotide translocators (ANTs) are mitochondrial carrier proteins that exchange ATP and ADP between the cytoplasm and the mitochondrial matrix. Four isoforms of ANT were identified: ANT1 (encoded by SLC25A4) is highly expressed in different tissues; ANT2 (encoded by SLC25A5) is predominantly expressed in proliferating, regenerating, or undifferentiated cells; ANT3 is ubiquitously expressed; ANT4 is mainly expressed in testis and germ cells. This antibody recognizes both of ANT1 and ANT2. The predicted molecular weight of ANT1/2 protein is around 32 kDa. While 60 kDa protein can also be observed, which has been proposed as the dimer of ANT1/2. (22423112)
实验方案
Product Specific Protocols | |
---|---|
WB protocol for CL Plus 750 SLC25A5 antibody CL750-83104 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |