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验证数据展示

  • WB analysis using 67700-1-Ig

    WB analysis using 67700-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67700-1-Ig (SLC6A12 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Caco-2 using 67700-1-Ig

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • IF Staining of Caco-2 using 67700-1-Ig

    IF Staining of Caco-2 using 67700-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using SLC6A12 antibody (67700-1-Ig, Clone: 2C1G4 ) at dilution of 1:800 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L).

SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67700-1-Ig

产品说明书

CloneNo. 2C1G4
引用文献(2)

宿主/亚型

Mouse / IgG2a

种属反应性

Human, Mouse, Rat and More (2)

应用

WB, IF/ICC, ELISA

别名

BGT 1, BGT1, FLJ38727, SLC6A12

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inL02 cells, rat liver tissue, HSC-T6 cells, HuH-7 cells, HepG2 cells, mouse hepatocytes
Positive IF/ICC detected inCaco-2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

67700-1-Ig targets SLC6A12 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse, Rat
文献引用反应性mouse, canine
免疫原 SLC6A12 fusion protein Ag18962 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
别名 BGT 1, BGT1, FLJ38727, SLC6A12
计算分子量 614 aa, 69 kDa
观测分子量70 kDa
GenBank蛋白编号BC126215
基因名称 SLC6A12
Gene ID (NCBI) 6539
RRIDAB_2882892
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48065
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SLC6A12 antibody 67700-1-IgDownload protocol
IF protocol for SLC6A12 antibody 67700-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
canineWB

Am J Physiol Renal Physiol

Mutation of a single threonine in the cytoplasmic NH2 terminus disrupts trafficking of renal betaine-GABA transporter 1 during hypertonic stress.

Authors - Eva S Schweikhard
View Article
mouseWB

Int J Biol Sci

Sodium chloride promotes macrophage pyroptosis and aggravates rheumatoid arthritis by activating SGK1 through GABA receptors Slc6a12

Authors - Xianzheng Zhang
View Article