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CoraLite® Plus 488-conjugated SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2C1G4

Cat no : CL488-67700

Print datasheet

Synonyms

Sodium- and chloride-dependent betaine transporter, Na(+)/Cl(-) betaine/GABA transporter, BGT-1, BGT1, BGT 1

验证数据展示

  • IF Staining of Caco-2 using CL488-67700

    IF Staining of Caco-2 using CL488-67700

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 SLC6A12 antibody (CL488-67700, Clone: 2C1G4 ) at dilution of 1:200.

  • FC experiment of MDA-MB-231 using CL488-67700

    FC experiment of MDA-MB-231 using CL488-67700

    1X10^6 MDA-MB-231 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SLC6A12 (CL488-67700, Clone:2C1G4) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inMDA-MB-231 cells
For other applications, we recommend the unconjugated version of this antibody, 67700-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67700 targets SLC6A12 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SLC6A12 fusion protein Ag18962 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
Synonyms Sodium- and chloride-dependent betaine transporter, Na(+)/Cl(-) betaine/GABA transporter, BGT-1, BGT1, BGT 1
Calculated Molecular Weight 614 aa, 69 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberBC126215
Gene Symbol SLC6A12
Gene ID (NCBI) 6539
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP48065
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLC6A12 antibody CL488-67700Download protocol
Standard Protocols
Click here to view our Standard Protocols