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验证数据展示

  • IF Staining of Caco-2 using CL488-67700

    IF Staining of Caco-2 using CL488-67700

    Immunofluorescent analysis of (-20°C Ethanol) fixed Caco-2 cells using CoraLite® Plus 488 SLC6A12 antibody (CL488-67700, Clone: 2C1G4 ) at dilution of 1:200.

  • FC experiment of MDA-MB-231 using CL488-67700

    FC experiment of MDA-MB-231 using CL488-67700

    1X10^6 MDA-MB-231 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human SLC6A12 (CL488-67700, Clone:2C1G4) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated SLC6A12 Monoclonal antibody

SLC6A12 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67700

产品说明书

CloneNo. 2C1G4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Sodium- and chloride-dependent betaine transporter, Na(+)/Cl(-) betaine/GABA transporter, BGT-1, BGT1, BGT 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inCaco-2 cells
Positive FC (Intra) detected inMDA-MB-231 cells
For other applications, we recommend the unconjugated version of this antibody, 67700-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67700 targets SLC6A12 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 SLC6A12 fusion protein Ag18962 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12
别名 Sodium- and chloride-dependent betaine transporter, Na(+)/Cl(-) betaine/GABA transporter, BGT-1, BGT1, BGT 1
计算分子量 614 aa, 69 kDa
观测分子量70 kDa
GenBank蛋白编号BC126215
基因名称 SLC6A12
Gene ID (NCBI) 6539
RRIDAB_3084373
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP48065
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SLC6A12 antibody CL488-67700Download protocol
Standard Protocols
Click here to view our Standard Protocols