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SMNDC1 Monoclonal antibody

SMNDC1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1B10C2

Cat no : 68344-1-Ig

Print datasheet

Synonyms

Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein

验证数据展示

  • WB analysis using 68344-1-Ig

    WB analysis using 68344-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68344-1-Ig (SMNDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HeLa using 68344-1-Ig

    FC experiment of HeLa using 68344-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SMNDC1 Monoclonal antibody (68344-1-Ig, Clone: 1B10C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, Daudi cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

68344-1-Ig targets SMNDC1 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen SMNDC1 fusion protein Ag33140 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name survival motor neuron domain containing 1
Synonyms Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein
Calculated Molecular Weight 238 aa, 27 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC011234
Gene Symbol SMNDC1
Gene ID (NCBI) 10285
RRIDAB_3085068
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75940
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).

实验方案

Product Specific Protocols
WB protocol for SMNDC1 antibody 68344-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Cancer

Two isoleucyl tRNAs that decode synonymous codons divergently regulate breast cancer metastatic growth by controlling translation of proliferation-regulating genes

Authors - Lisa B Earnest-Noble
View Article