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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68344-1-Ig (same clone as 68344-1-PBS)

    WB analysis using 68344-1-Ig (same clone as 68344-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68344-1-Ig (SMNDC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68344-1-Ig (same clone as 68344-1-PBS)

    FC experiment of HeLa using 68344-1-Ig (same clone as 68344-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SMNDC1 Monoclonal antibody (68344-1-Ig, Clone: 1B10C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1) (red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68344-1-PBS in a different storage buffer formulation.

SMNDC1 Monoclonal antibody, PBS Only

SMNDC1 Monoclonal Antibody for Indirect ELISA, WB
Cat No. 68344-1-PBS

产品说明书

CloneNo. 1B10C2

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

Indirect ELISA, WB

别名

Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

68344-1-PBS targets SMNDC1 in Indirect ELISA, WB applications and shows reactivity with human samples.

经测试应用 Indirect ELISA, WB Application Description
经测试反应性 human
免疫原 SMNDC1 fusion protein Ag33140 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 survival motor neuron domain containing 1
别名 Survival motor neuron domain-containing protein 1, SPF30, SMN-related protein, SMNR, SMN related protein
计算分子量 238 aa, 27 kDa
观测分子量 32 kDa
GenBank蛋白编号BC011234
基因名称 SMNDC1
Gene ID (NCBI) 10285
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDO75940
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Survival motor neuron domain-containing protein 1 (SMNDC1), also known as survival of motor neuron-related-splicing factor 30 (SPF30) or SMN-related protein (SMNR), is a constituent of the spliceosome complex that plays an essential role in spliceosome assembly by recruiting U4/U5/U6 tri-snRNP to the pre-spliceosomal complex (PMID: 9817934; 11331595; 30904945). Both SMNDC1 and SMN1 contain a highly conserved central Tudor domain which functions as a protein-protein interaction surface and mediates binding to the spliceosomal Sm proteins (PMID: 11331595).