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验证数据展示

  • IF Staining of MDCK using 66851-2-Ig

    IF Staining of MDCK using 66851-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using SMO antibody (66851-2-Ig, Clone: 2A11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC, ELISA
Cat No. 66851-2-Ig

产品说明书

CloneNo. 2A11A10

宿主/亚型

Mouse / IgG1

种属反应性

Human, Canine

应用

IF/ICC, ELISA

别名

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66851-2-Ig targets SMO in IF/ICC, ELISA applications and shows reactivity with Human, Canine samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 Human, Canine
免疫原 SMO fusion protein Ag25861 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 smoothened homolog (Drosophila)
别名 Gx, Protein Gx, SMO, SMOH, Smoothened homolog
计算分子量 787 aa, 86 kDa
GenBank蛋白编号BC009989
基因名称 SMO
Gene ID (NCBI) 6608
RRIDAB_3085033
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ99835
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

实验方案

Product Specific Protocols
IF protocol for SMO antibody 66851-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols