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验证数据展示

  • IF Staining of MDCK using 66851-2-Ig

    IF Staining of MDCK using 66851-2-Ig

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using SMO antibody (66851-2-Ig, Clone: 2A11A10 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), ARL13B antibody (17711-1-AP, red).

SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC, ELISA
Cat No. 66851-2-Ig

Print datasheet

CloneNo. 2A11A10

Host / Isotype

Mouse / IgG1

Reactivity

Human, Canine

Applications

IF/ICC, ELISA

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMDCK cells
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66851-2-Ig targets SMO in IF/ICC, ELISA applications and shows reactivity with Human, Canine samples.

Tested Applications IF/ICC, ELISA Application Description
Tested Reactivity Human, Canine
Immunogen SMO fusion protein Ag25861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name smoothened homolog (Drosophila)
Synonyms Gx, Protein Gx, SMO, SMOH, Smoothened homolog
Calculated Molecular Weight 787 aa, 86 kDa
GenBank Accession NumberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
RRIDAB_3085033
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99835
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

实验方案

Product Specific Protocols
IF protocol for SMO antibody 66851-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols