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验证数据展示

  • IF Staining of hTERT-RPE1 using CL488-66851-2

    IF Staining of hTERT-RPE1 using CL488-66851-2

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using CoraLite® Plus 488 SMO antibody (CL488-66851, Clone: 2B10A11 ) at dilution of 1:200, ARL13B antibody (17711-1-AP, red).

CoraLite® Plus 488-conjugated SMO Monoclonal antibody

SMO Monoclonal Antibody for IF/ICC
Cat No. CL488-66851-2

Print datasheet

CloneNo. 2A11A10

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

IF/ICC

Synonyms

Gx, Protein Gx, SMO, SMOH, Smoothened homolog

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inhTERT-RPE1 cells
Planning an IHC experiment? We recommend our IHCeasy SMO Ready-To-Use IHC Kit. SMO primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66851-2-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66851-2 targets SMO in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen SMO fusion protein Ag25861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name smoothened homolog (Drosophila)
Synonyms Gx, Protein Gx, SMO, SMOH, Smoothened homolog
Calculated Molecular Weight 787 aa, 86 kDa
GenBank Accession NumberBC009989
Gene Symbol SMO
Gene ID (NCBI) 6608
RRIDAB_3084278
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ99835
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Smoothened (SMO) is a G protein-coupled receptor that is a component of the hedgehog (Hh) signaling pathway and is conserved from flies to humans. The Hh pathway plays central roles in animal development and stem-cell function. Defects in Hh signalling lead to birth defects and cancer in humans. Expressed on the primary cilium, SMO interacts with the patched protein, a receptor for Hh proteins.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SMO antibody CL488-66851-2Download protocol
Standard Protocols
Click here to view our Standard Protocols