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验证数据展示

  • IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

SMURF2 Recombinant antibody, PBS Only

SMURF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84445-1-PBS

Print datasheet

CloneNo. 241486F3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Synonyms

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMURF2 Ready-To-Use IHC Kit. SMURF2 primary antibody included.

产品信息

84445-1-PBS targets SMURF2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human
Immunogen SMURF2 fusion protein Ag33419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name SMAD specific E3 ubiquitin protein ligase 2
Synonyms SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2
Calculated Molecular Weight 748 aa, 86 kDa
GenBank Accession NumberBC093876
Gene Symbol SMURF2
Gene ID (NCBI) 64750
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9HAU4
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).