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验证数据展示

  • IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    IF Staining of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

  • FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    FC experiment of U2OS using 84445-1-RR (same clone as 84445-1-PBS)

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84445-1-PBS in a different storage buffer formulation.

SMURF2 Recombinant antibody, PBS Only

SMURF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84445-1-PBS

产品说明书

CloneNo. 241486F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), Indirect ELISA

别名

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy SMURF2 Ready-To-Use IHC Kit. SMURF2 primary antibody included.

产品信息

84445-1-PBS targets SMURF2 in IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原 SMURF2 fusion protein Ag33419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD specific E3 ubiquitin protein ligase 2
别名 SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2
计算分子量 748 aa, 86 kDa
GenBank蛋白编号BC093876
基因名称 SMURF2
Gene ID (NCBI) 64750
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HAU4
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).