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验证数据展示

  • IF Staining of U2OS using 84445-1-RR

    IF Staining of U2OS using 84445-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • IF Staining of U2OS using 84445-1-RR

    IF Staining of U2OS using 84445-1-RR

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SMURF2 antibody (84445-1-RR, Clone: 241486F3 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • FC experiment of U2OS using 84445-1-RR

    FC experiment of U2OS using 84445-1-RR

    1x10^6 U2OS cells were intracellularly stained with 0.25 ug SMURF2 Recombinant antibody (84445-1-RR, Clone:241486F3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

SMURF2 Recombinant antibody

SMURF2 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 84445-1-RR

产品说明书

CloneNo. 241486F3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC (Intra), ELISA

别名

SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy SMURF2 Ready-To-Use IHC Kit. SMURF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84445-1-RR targets SMURF2 in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原 SMURF2 fusion protein Ag33419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 SMAD specific E3 ubiquitin protein ligase 2
别名 SMAD-specific E3 ubiquitin-protein ligase 2, hSMURF2, HECT-type E3 ubiquitin transferase SMURF2, EC:2.3.2.26, E3 ubiquitin-protein ligase SMURF2
计算分子量 748 aa, 86 kDa
GenBank蛋白编号BC093876
基因名称 SMURF2
Gene ID (NCBI) 64750
RRIDAB_3671970
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ9HAU4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SMURF2 is a HECT domain E3 ubiquitin ligase involved in degradation of SMADs,TGF-beta receptor,and other substrates.It also functions in regulation of neuronal and planar cell polarity, induction of senescence, and tumor suppression(PMID:22231558).SMURF2 associates constitutively with SMAD7 and it is nuclear, but binding to SMAD7 induces export and recruitment to activated TGFBR, where it causes degradation of receptors and of SMAD7 via proteasomal and lysosomal pathways(PMIF:11163210).

实验方案

Product Specific Protocols
IF protocol for SMURF2 antibody 84445-1-RRDownload protocol
FC protocol for SMURF2 antibody 84445-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols