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SNRPB2 Monoclonal antibody

SNRPB2 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1D11E9

Cat no : 68095-1-Ig

Print datasheet

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

验证数据展示

  • WB analysis using 68095-1-Ig

    WB analysis using 68095-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • FC experiment of HeLa using 68095-1-Ig

    FC experiment of HeLa using 68095-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive FC (Intra) detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68095-1-Ig targets SNRPB2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen SNRPB2 fusion protein Ag5540 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide B''
Synonyms U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9
Calculated Molecular Weight 225 aa, 26, 5 kDa
Observed Molecular Weight25-31 kDa
GenBank Accession NumberBC036737
Gene Symbol SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2918832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08579
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.

实验方案

Product Specific Protocols
WB protocol for SNRPB2 antibody 68095-1-IgDownload protocol
FC protocol for SNRPB2 antibody 68095-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols