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SNRPB2 Monoclonal antibody, PBS Only

SNRPB2 Monoclonal Antibody for Indirect ELISA, WB

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

Indirect ELISA, WB

Conjugate

Unconjugated

CloneNo.

1D11E9

Cat no : 68095-1-PBS

Print datasheet

Synonyms

U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9

验证数据展示

  • WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

    WB analysis using 68095-1-Ig (same clone as 68095-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68095-1-Ig (SNRPB2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

    FC experiment of HeLa using 68095-1-Ig (same clone as 68095-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug SNRPB2 Monoclonal antibody (68095-1-Ig, Clone:1D11E9) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 68095-1-PBS in a different storage buffer formulation.

产品信息

68095-1-PBS targets SNRPB2 in Indirect ELISA, WB applications and shows reactivity with human samples.

Tested Applications Indirect ELISA, WB Application Description
Tested Reactivity human
Immunogen SNRPB2 fusion protein Ag5540 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name small nuclear ribonucleoprotein polypeptide B''
Synonyms U2 snRNP B'', U2 snRNP B, U2 small nuclear ribonucleoprotein B'', 1D11E9
Calculated Molecular Weight 225 aa, 26, 5 kDa
Observed Molecular Weight25-31 kDa
GenBank Accession NumberBC036737
Gene Symbol SNRPB2
Gene ID (NCBI) 6629
RRIDAB_2918832
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08579
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

U2 small nuclear ribonucleoprotein B''(SNRPB2) is part of the U2 RNP particle, which associated with the branch point of the lariat-shaped intron, in intermediate in the cascade of mRNA precursor splicing event. Only in presence of the U2A' protein, SNRPB2 binds stem loop IV of U2 snRNA.