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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated SOD2 Monoclonal antibody

SOD2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

3A6C2

Cat no : CL555-66474

Print datasheet

Synonyms

SODM, MNSOD, Mn SOD, IPOB, IPO B

验证数据展示

  • FC experiment of HeLa using CL555-66474

    FC experiment of HeLa using CL555-66474

    1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite®555 Anti-Human SOD2 (CL555-66474, Clone:3A6C2) (red), or 0.8 ug CoraLite® Plus 555 Mouse IgG2a Isotype Control (C1.18.4) (CL555-65208, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of A549 using CL555-66474

    IF Staining of A549 using CL555-66474

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®555 SOD2 antibody (CL555-66474, Clone: 3A6C2 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66474-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-66474 targets SOD2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen SOD2 fusion protein Ag21388 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name superoxide dismutase 2, mitochondrial
Synonyms SODM, MNSOD, Mn SOD, IPOB, IPO B
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC016934
Gene Symbol SOD2
Gene ID (NCBI) 6648
RRIDAB_2919686
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP04179
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.

实验方案

Product Specific Protocols
IF protocol for CL555 SOD2 antibody CL555-66474Download protocol
Standard Protocols
Click here to view our Standard Protocols