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CoraLite® Plus 647-conjugated SOD2 Monoclonal antibody
SOD2 Monoclonal Antibody for IF/ICC
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse, rat, pig
Applications
IF/ICC
Conjugate
CoraLite® Plus 647 Fluorescent Dye
CloneNo.
3A6C2
验证数据展示
经过测试的应用
Positive IF/ICC detected in | A549 cells |
Planning an IHC experiment? We recommend our IHCeasy SOD2 Ready-To-Use IHC Kit. SOD2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66474-1-Ig
推荐稀释比
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL647-66474 targets SOD2 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.
Tested Applications | IF/ICC Application Description |
Tested Reactivity | human, mouse, rat, pig |
Immunogen | SOD2 fusion protein Ag21388 种属同源性预测 |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Full Name | superoxide dismutase 2, mitochondrial |
Synonyms | SODM, MNSOD, Mn SOD, IPOB, IPO B |
Calculated Molecular Weight | 25 kDa |
Observed Molecular Weight | 25 kDa |
GenBank Accession Number | BC016934 |
Gene Symbol | SOD2 |
Gene ID (NCBI) | 6648 |
RRID | AB_2920285 |
Conjugate | CoraLite® Plus 647 Fluorescent Dye |
Excitation/Emission Maxima Wavelengths | 654 nm / 674 nm |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P04179 |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 647 SOD2 antibody CL647-66474 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |