验证数据展示
经过测试的应用
Positive IF-P detected in | human colon cancer tissue |
Positive IF/ICC detected in | HEK-293 cells, human colon cancer tissue |
Positive FC (Intra) detected in | HEK-293 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66508 targets SP1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.
经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
经测试反应性 | human, mouse, rat |
免疫原 | SP1 fusion protein Ag16720 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | Sp1 transcription factor |
别名 | TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3 |
计算分子量 | 785 aa, 81 kDa |
观测分子量 | 95-105 kDa, 60-65 kDa |
GenBank蛋白编号 | BC062539 |
基因名称 | SP1 |
Gene ID (NCBI) | 6667 |
ENSEMBL Gene ID | ENSG00000185591 |
RRID | AB_3084253 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein G purification |
UNIPROT ID | P08047 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].
实验方案
Product Specific Protocols | |
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IF protocol for CL Plus 488 SP1 antibody CL488-66508 | Download protocol |
FC protocol for CL Plus 488 SP1 antibody CL488-66508 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |