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CoraLite®594-conjugated SP1 Monoclonal antibody

SP1 Monoclonal Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

4G3H3

Cat no : CL594-66508

Print datasheet

Synonyms

TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3

验证数据展示

  • IF Staining of human colon cancer using CL594-66508

    IF Staining of human colon cancer using CL594-66508

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

  • IF Staining of HEK-293 using CL594-66508

    IF Staining of HEK-293 using CL594-66508

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®594 SP1 antibody (CL594-66508, Clone: 4G3H3 ) at dilution of 1:200.

  • FC experiment of HEK-293 using CL594-66508

    FC experiment of HEK-293 using CL594-66508

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human SP1 (CL594-66508, Clone:4G3H3) (red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF-P detected inhuman colon cancer tissue
Positive IF/ICC detected inHEK-293 cells, human colon cancer tissue
Positive FC (Intra) detected inHEK-293 cells
Planning an IHC experiment? We recommend our IHCeasy SP1 Ready-To-Use IHC Kit. SP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66508-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66508 targets SP1 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IF-P, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen SP1 fusion protein Ag16720 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name Sp1 transcription factor
Synonyms TSFP1, Specificity protein 1, Sp1 transcription factor, SP 1, 4G3H3
Calculated Molecular Weight 785 aa, 81 kDa
Observed Molecular Weight 95-105 kDa, 60-65 kDa
GenBank Accession NumberBC062539
Gene Symbol SP1
Gene ID (NCBI) 6667
ENSEMBL Gene IDENSG00000185591
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP08047
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The transcription factor Sp1 is a C2H2 zinc-finger protein that is involved in the regulation of a wide variety of genes, including housekeeping genes and tumor-developing genes. It is associated with tumor development, growth, and metastasis [PMID: 16209919]. It regulates the expression of a large number of genes involved in a variety of processes such as cell growth, apoptosis, differentiation and immune responses. Besides, it has a role in modulating the cellular response to DNA damage, recruiting SMARCA4/BRG1 on the c-FOS promoter, regulation of FE65 gene expression [PMID:11371615,16332679]. SP1 has some isoforms with MW ~95-106 kDa and ~60 kDa [PMID:17462816].

实验方案

Product Specific Protocols
IF protocol for CL594 SP1 antibody CL594-66508Download protocol
FC protocol for CL594 SP1 antibody CL594-66508Download protocol
Standard Protocols
Click here to view our Standard Protocols