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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated SPAG4L Polyclonal antibody

SPAG4L Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-17495

Print datasheet

Synonyms

dJ726C3.1, SPAG4L, SUN5, TSARG4

验证数据展示

  • FC experiment of PC-3 using CL488-17495

    FC experiment of PC-3 using CL488-17495

    1X10^6 PC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SPAG4L (CL488-17495) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive FC (Intra) detected inPC-3 cells
Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 17495-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-17495 targets SPAG4L in FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen SPAG4L fusion protein Ag11629 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name sperm associated antigen 4-like
Synonyms dJ726C3.1, SPAG4L, SUN5, TSARG4
Calculated Molecular Weight 379 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC026118
Gene Symbol SPAG4L
Gene ID (NCBI) 140732
RRIDAB_2919150
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8TC36
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 SPAG4L antibody CL488-17495Download protocol
Standard Protocols
Click here to view our Standard Protocols