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验证数据展示

  • FC experiment of PC-3 using CL594-17495

    FC experiment of PC-3 using CL594-17495

    1X10^6 PC-3 cells were intracellularly stained with 0.8 ug CoraLite®594 Anti-Human SPAG4L (CL594-17495) (red), or 0.8 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated SPAG4L Polyclonal antibody

SPAG4L Polyclonal Antibody for FC (Intra)
Cat No. CL594-17495

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

dJ726C3.1, SPAG4L, SUN5, TSARG4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive FC (Intra) detected inPC-3 cells
Positive FC detected inPC-3 cells
For other applications, we recommend the unconjugated version of this antibody, 17495-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-17495 targets SPAG4L in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 SPAG4L fusion protein Ag11629 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 4-like
别名 dJ726C3.1, SPAG4L, SUN5, TSARG4
计算分子量 379 aa, 43 kDa
观测分子量 43 kDa
GenBank蛋白编号BC026118
基因名称 SPAG4L
Gene ID (NCBI) 140732
RRIDAB_2919843
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8TC36
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG4L,also named as SUN domain-containing protein 5 or Testis and spermatogenesis-related gene 4 protein, is a 379 amino acid protein, which contains 1 SUN domain. SPAG4L is widely expressed and localizes in the nucleus inner membrane. SPAG4L is restricted to the apical nuclear region of round spermatids that face the acrosomic vesicle. SPAG4L is involved in linkage of the acrosomic vesicle to the spermatid nucleus, and in acrosome biogenesis.

实验方案

Product Specific Protocols
FC protocol for CL594 SPAG4L antibody CL594-17495Download protocol
Standard Protocols
Click here to view our Standard Protocols