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验证数据展示

  • IF Staining of HeLa using CL488-14726

    IF Staining of HeLa using CL488-14726

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 SPAG5 antibody (CL488-14726) at dilution of 1:200.

  • FC experiment of HeLa using CL488-14726

    FC experiment of HeLa using CL488-14726

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human SPAG5 (CL488-14726) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated SPAG5 Polyclonal antibody

SPAG5 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-14726

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, FC (Intra)

别名

sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 14726-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-14726 targets SPAG5 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse
免疫原 SPAG5 fusion protein Ag6485 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 sperm associated antigen 5
别名 sperm associated antigen 5, Mitotic spindle-associated protein p126, MAP126, hMAP126, Deepest
计算分子量 134 kDa
观测分子量 135 kDa, 150 kDa
GenBank蛋白编号BC000322
基因名称 SPAG5
Gene ID (NCBI) 10615
RRIDAB_2919100
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96R06
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

SPAG5, also named as MAP126, Astrin and Deepest, is necessary for normal spindle formation during mitosis. It is highly expressed in testis. SPAG5 has different localization in somatic versus male germ cells suggesting the possibility of different function. This antibody is specific to SPAG5.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SPAG5 antibody CL488-14726Download protocol
FC protocol for CL Plus 488 SPAG5 antibody CL488-14726Download protocol
Standard Protocols
Click here to view our Standard Protocols