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CoraLite® Plus 488-conjugated SRA1 Polyclonal antibody

SRA1 Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-24655

Print datasheet

Synonyms

Steroid receptor RNA activator protein, Steroid receptor RNA activator 1, SRAP, PP7684

验证数据展示

  • FC experiment of MCF-7 using CL488-24655

    FC experiment of MCF-7 using CL488-24655

    1x10^6 MCF-7 cells were intracellularly stained with 0.8 ug Coralite® Plus 488 Sra1 Polyclonal Antibody (CL488-24655)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of MCF-7 using CL488-24655

    IF Staining of MCF-7 using CL488-24655

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 SRA1 antibody (CL488-24655) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy SRA1 Ready-To-Use IHC Kit. SRA1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 24655-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-24655 targets SRA1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse
Immunogen SRA1 fusion protein Ag20082 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name steroid receptor RNA activator 1
Synonyms Steroid receptor RNA activator protein, Steroid receptor RNA activator 1, SRAP, PP7684
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 30 kDa, 33 kDa
GenBank Accession NumberBC067895
Gene Symbol SRA1
Gene ID (NCBI) 10011
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9HD15
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription. This polyclonal antibody recognizes endogenous SRA1 isoforms which migrate as a doublet on SDS-PAGE gels (PMID: 12565891; 23907597).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 SRA1 antibody CL488-24655Download protocol
FC protocol for CL Plus 488 SRA1 antibody CL488-24655Download protocol
Standard Protocols
Click here to view our Standard Protocols