SRP19 Recombinant antibody
SRP19 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
241219E9
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, A549 cells, K-562 cells, Raji cells, mouse kidney tissue, mouse ovary tissue, mouse liver tissue |
| Positive IF/ICC detected in | HeLa cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
The immunogen of 84061-3-RR is SRP19 Fusion Protein expressed in E. coli.
| Tested Applications | WB, IF/ICC, ELISA Application Description |
| Tested Reactivity | human, mouse |
| Immunogen | SRP19 fusion protein Ag8903 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Full Name | signal recognition particle 19kDa |
| Synonyms | 241219E9 |
| Calculated Molecular Weight | 144 aa, 16 kDa |
| Observed Molecular Weight | 17 kDa |
| GenBank Accession Number | BC010947 |
| Gene Symbol | SRP19 |
| Gene ID (NCBI) | 6728 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P09132 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The signal recognition particle (SRP) is one of the few functional small RNP particles. The SRP couples the synthesis of membrane and secretory proteins across or into the endoplasmic reticulum (ER) membrane in eukaryotes, as well as across the bacterial plasma membrane, and chloroplast thylakoid membranes. The mammalian SRP is composed of a 7S (or 7SL) RNA and six different proteins, SRP9, SRP14, SRP19, SRP54, SRP68 and SRP72. All of the components of SRP, including SRP RNA, participate directly in the overall protein targeting process. SRP19 binds directly to 7S RNA and mediates binding of the 54 kDa subunit of the SRP. SRP19 was shown to significantly enhance SRP54 attachment to helix 8 of 7SL RNA.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for SRP19 antibody 84061-3-RR | Download protocol |
| IF protocol for SRP19 antibody 84061-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |