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STAM Monoclonal antibody

STAM Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1F5C2

Cat No : 68628-1-Ig

Print datasheet

Synonyms

DKFZp686J2352, STAM, STAM 1, STAM1

验证数据展示

  • WB analysis using 68628-1-Ig

    WB analysis using 68628-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of RAW 264.7 using 68628-1-Ig

    WB analysis of RAW 264.7 using 68628-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHCT 116 cells, RAW 264.7 cells, HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68628-1-Ig targets STAM in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen STAM fusion protein Ag33192 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
Synonyms DKFZp686J2352, STAM, STAM 1, STAM1
Calculated Molecular Weight 59 kDa
Observed Molecular Weight 70 kDa
GenBank Accession NumberBC030586
Gene Symbol STAM
Gene ID (NCBI) 8027
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ92783
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

实验方案

Product Specific Protocols
WB protocol for STAM antibody 68628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols