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验证数据展示

  • WB analysis using 68628-1-Ig

    WB analysis using 68628-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • WB analysis of RAW 264.7 using 68628-1-Ig

    WB analysis of RAW 264.7 using 68628-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 68628-1-Ig (STAM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

STAM Monoclonal antibody

STAM Monoclonal Antibody for WB, ELISA
Cat No. 68628-1-Ig

产品说明书

CloneNo. 1F5C2

宿主/亚型

Mouse / IgG2b

种属反应性

Human, mouse, rat

应用

WB, ELISA

别名

DKFZp686J2352, STAM, STAM 1, STAM1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHCT 116 cells, RAW 264.7 cells, HeLa cells, LNCaP cells, HepG2 cells, Jurkat cells, K-562 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68628-1-Ig targets STAM in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 STAM fusion protein Ag33192 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 signal transducing adaptor molecule (SH3 domain and ITAM motif) 1
别名 DKFZp686J2352, STAM, STAM 1, STAM1
计算分子量 59 kDa
观测分子量 70 kDa
GenBank蛋白编号BC030586
基因名称 STAM
Gene ID (NCBI) 8027
RRIDAB_3085320
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ92783
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAM, also named as STAM1, belongs to the STAM family. It is involved in intracellular signal transduction mediated by cytokines and growth factors. Upon IL-2 and GM-CSL stimulation, it plays a role in signaling leading to DNA synthesis and MYC induction. STAM may also play a role in T-cell development. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs). Together with HRS, STAM forms ESCRT-0. STAM was originally identified as an adaptor protein involved in cytokine signaling.(PMID:20505072)

实验方案

Product Specific Protocols
WB protocol for STAM antibody 68628-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols