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  • Featured Product
  • KD/KO Validated

STAT1 Recombinant antibody, PBS Only

STAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F16

Cat no : 82016-1-PBS

Print datasheet

Synonyms

Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16

验证数据展示

  • WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82016-1-RR

    Affinity and Kinetic Characterization of 82016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

产品信息

82016-1-PBS targets STAT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STAT1 fusion protein Ag0199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42224
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.