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  • Featured Product
  • KD/KO Validated

STAT1 Recombinant antibody, PBS Only

STAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2F16

Cat no : 82016-1-PBS

Print datasheet

Synonyms

DKFZp686B04100, ISGF 3, STAT1, STAT91

验证数据展示

  • WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis using 82016-1-RR (same clone as 82016-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    IHC staining of human ovary tumor using 82016-1-RR (same clone as 82016-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STAT1 antibody included
  • WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB analysis of HEK-293 using 82016-1-RR (same clone as 82016-1-PBS)

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP experiment of PC-3 using 82016-1-RR (same clone as 82016-1-PBS)

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug. This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    IF Staining of HeLa using 82016-1-RR (same clone as 82016-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82016-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82016-1-PBS

    Affinity and Kinetic Characterization of 82016-1-PBS

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

产品信息

82016-1-PBS targets STAT1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen STAT1 fusion protein Ag0199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms DKFZp686B04100, ISGF 3, STAT1, STAT91
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42224
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.