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  • Featured Product
  • KD/KO Validated

STAT1 Recombinant antibody

STAT1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2F16

Cat no : 82016-1-RR

Print datasheet

Synonyms

Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16

验证数据展示

  • WB analysis using 82016-1-RR

    WB analysis using 82016-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82016-1-RR (STAT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 82016-1-RR

    IHC staining of human ovary tumor using 82016-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT1 antibody included
  • IHC staining of human ovary tumor using 82016-1-RR

    IHC staining of human ovary tumor using 82016-1-RR

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 82016-1-RR (STAT1 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STAT1 antibody included
  • WB analysis of HEK-293 using 82016-1-RR

    WB analysis of HEK-293 using 82016-1-RR

    WB result of STAT1 antibody (82016-1-RR; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-STAT1 transfected HEK-293 cells.

  • IP experiment of PC-3 using 82016-1-RR

    IP experiment of PC-3 using 82016-1-RR

    IP result of anti-STAT1 (IP:82016-1-RR, 4ug; Detection:82016-1-RR 1:10000) with PC-3 cells lysate 2000 ug.

  • IF Staining of HeLa using 82016-1-RR

    IF Staining of HeLa using 82016-1-RR

    Immunofluorescent analysis of (4% PFA) fixed IFN alpha treated HeLa cells using STAT1 antibody (82016-1-RR, Clone: 2F16 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

  • Affinity and Kinetic Characterization of 82016-1-RR

    Affinity and Kinetic Characterization of 82016-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82016-1-RR against Human STAT1 were performed. The affinity constant is 0.501 nM.

经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, A549 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells
Positive IP detected inPC-3 cells
Positive IHC detected inhuman ovary tumor tissue, mouse spleen tissue, mouse colon tissue, rat colon tissue, rat spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inIFN alpha treated HeLa cells
Planning an IHC experiment? We recommend our IHCeasy STAT1 Ready-To-Use IHC Kit. STAT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82016-1-RR targets STAT1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen STAT1 fusion protein Ag0199 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name signal transducer and activator of transcription 1, 91kDa
Synonyms Transcription factor ISGF-3 components p91/p84, STAT91, Signal transducer and activator of transcription 1-alpha/beta, ISGF 3, 2F16
Calculated Molecular Weight 83 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC002704
Gene Symbol STAT1
Gene ID (NCBI) 6772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42224
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STAT1 (signal transducers and activators of transcription 1) is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. Various ligands, including interferons- and - , EGF, PDGF and IL-6, can activate STAT1. STAT1 protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens.

实验方案

Product Specific Protocols
WB protocol for STAT1 antibody 82016-1-RRDownload protocol
IHC protocol for STAT1 antibody 82016-1-RRDownload protocol
IF protocol for STAT1 antibody 82016-1-RRDownload protocol
IP protocol for STAT1 antibody 82016-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols