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STIP1 Monoclonal antibody, PBS Only

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B5H2

Cat no : 68155-1-PBS

Print datasheet

Synonyms

HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1

验证数据展示

  • WB analysis using 68155-1-Ig (same clone as 68155-1-PBS)

    WB analysis using 68155-1-Ig (same clone as 68155-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    IP experiment of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STIP1 antibody included
  • IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    IHC staining of mouse liver using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with STIP1 antibody included
  • IF Staining of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    IF Staining of HeLa using 68155-1-Ig (same clone as 68155-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68155-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy STIP1 Ready-To-Use IHC Kit. STIP1 primary antibody included.

产品信息

68155-1-PBS targets STIP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen STIP1 fusion protein Ag7851 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name stress-induced-phosphoprotein 1
Synonyms HOP, Hsc70/Hsp90 organizing protein, IEF SSP 3521, P60, STI1, STI1L, STIP1
Calculated Molecular Weight 63 kDa
Observed Molecular Weight61 kDa
GenBank Accession NumberBC002987
Gene Symbol STIP1
Gene ID (NCBI) 10963
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31948
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).