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验证数据展示

  • IHC staining of mouse liver using 68155-1-Ig

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STIP1 antibody included
  • IF Staining of HeLa using 68155-1-Ig

    IF Staining of HeLa using 68155-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using STIP1 antibody (68155-1-Ig, Clone: 1B5H2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • FC experiment of MCF-7 using 68155-1-Ig

    FC experiment of MCF-7 using 68155-1-Ig

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug STIP1 Monoclonal antibody (68155-1-Ig, Clone:1B5H2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • WB analysis using 68155-1-Ig

    WB analysis using 68155-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68155-1-Ig (STIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IP experiment of HeLa using 68155-1-Ig

    IP experiment of HeLa using 68155-1-Ig

    IP result of anti-STIP1 (IP:68155-1-Ig, 5ug; Detection:68155-1-Ig 1:1000) with HeLa cells lysate 1880 ug.

  • IHC staining of mouse liver using 68155-1-Ig

    IHC staining of mouse liver using 68155-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68155-1-Ig (STIP1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with STIP1 antibody included

STIP1 Monoclonal antibody

STIP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 68155-1-Ig

产品说明书

CloneNo. 1B5H2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

别名

Renal carcinoma antigen NY-REN-11, P60, IEF SSP 3521, Hsc70/Hsp90-organizing protein, Hsc70/Hsp90 organizing protein

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IP detected inHeLa cells, Jurkat cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy STIP1 Ready-To-Use IHC Kit. STIP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68155-1-Ig targets STIP1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 STIP1 fusion protein Ag7851 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 stress-induced-phosphoprotein 1
别名 Renal carcinoma antigen NY-REN-11, P60, IEF SSP 3521, Hsc70/Hsp90-organizing protein, Hsc70/Hsp90 organizing protein
计算分子量 63 kDa
观测分子量61 kDa
GenBank蛋白编号BC002987
基因名称 STIP1
Gene ID (NCBI) 10963
RRIDAB_2935260
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP31948
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

STIP1, also named as Hop and NY-REN-11. Its expression is low in the hypothalamus. STIP1 has some isoforms with MW 62 kDa, 68 kDa and 59 kDa. Acts as a co-chaperone for HSP90AA1.Mediates the association of the molecular chaperones HSPA8/HSC70 and HSP90, Probably forms a complex composed of chaperones HSP90 and HSP70, co-chaperones STIP1/HOP, CDC37, PPP5C, PTGES3/p23, TSC1 and client protein TSC2. STIP1 interacts with PACRG, EEF1AKMT3, FLCN, FNIP1 and FNIP2 (PMID:24880080).

实验方案

Product Specific Protocols
WB protocol for STIP1 antibody 68155-1-IgDownload protocol
IHC protocol for STIP1 antibody 68155-1-IgDownload protocol
IF protocol for STIP1 antibody 68155-1-IgDownload protocol
IP protocol for STIP1 antibody 68155-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols